An essential oil was extracted from the ripe seeds of highly medicinal indigenous plant Bryonia lacinosa. GLC and GLC-MS of the oil revealed altogether 10 components, the main constituents being palmitic acid (30.6%), stearic acid (22.9%) oleic acid (17.8%) linoleic acid (13.7%) and butyric acid (11.5%). The oil has been evaluated for the antimicrobial activity against pathogenic strains of Gram positive (Staphylococcus aureus) and Gram negative (Escherichia Coli, Pseudomonas aeruginosa) bacteria in vitro. It showed strong activity against Pseudomonas aeruginosa and Staphylococcus aureus. Seed oil with MBC concentration of 5 mg/ml inhibited the growth of Staphylococcus aureus, while for inhibiting the growth of Pseudomonas aeruginosa and Escherichia coli, the required MBC concentrations for the oil were 1.25 mg/ml and > 5 mg/ml respectively. These results show that Bryonia lacinosa seed oil could be used as a natural alternative to traditional food preservatives.


Bryonia lacinosa, essential oil, chemical composition, anti-bacterial activity

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